Effects of the vitamins A, C and E on the fluidity of the erythrocyte membranes from horse before and after exercise

Aim: The aim of present research was to examine in horses, "Italian Trotters", the rheological behavior of the lipid bilayer and TEARS determination, after intense physical exercise. The membrane fluidity was studied after the addition of reducing vitamins (E, C) in erythrocyte (RBC) membranes both from subjects not trained and those that per- formed standard physical activity. Exercise-induced oxidative processes are reflected in changes of blood constituents. Exercise is a powerful stimulator of ROS production. The concentrations of ROS are normally well controlled by mechanism usuful to prevent oxidative damages. The primary defense system consists of antioxidant compounds, such as ascorbic acid, vitamin E,along with a variety of antioxidant enzymes such as SQD, catalase, glutathione peroxidase. Methods: The fluidity of RBC membranes was determined by fluorescence polarization of DPH. The damages from free radicals in RBC are determined by the photometric method of Gilbert. Results: The Results showed how greater is the effectiveness of liposoluble and reducent vitamins on fluorescence polarization of RBC membranes in comparison to untreated preparations. Conclusion: Authors evidenced that the reducing vitamins (E and C), are very effective on the stability of RBC membranes. Determination of TBARS on RBC membranes confirm the previous data.