Oleic, linoleic and linolenic acids exert several beneficial effects on human health, some of which are also certified by recent European and U.S. regulations. The goal of the presented work was to develop an innovative methodology to evaluate their content in edible vegetable oils, in order to increase the value of oils from a nutraceutical perspective. The protocol is based on the use of paper spray ionization coupled with tandem mass spectrometry experiments, which allowed the recording of data very quickly and with high specificity. All investigated compounds gained a good linear relation (r2 higher than 0.98). Accuracy values are near 100% for all concentration levels examined, and the repeatability and reproducibility data result lower than 15%, highlighting the consistence of the methodology. The developed approach was successfully applied for the analysis of different real samples, and its robustness was confirmed by comparing the results obtained with those coming from the classical and official methodology.

Paper Spray Tandem Mass Spectrometry for Assessing Oleic, Linoleic and Linolenic Acid Content in Edible Vegetable Oils

Bartella L.;Mazzotti F.;Talarico I. R.;Di Donna L.
2023-01-01

Abstract

Oleic, linoleic and linolenic acids exert several beneficial effects on human health, some of which are also certified by recent European and U.S. regulations. The goal of the presented work was to develop an innovative methodology to evaluate their content in edible vegetable oils, in order to increase the value of oils from a nutraceutical perspective. The protocol is based on the use of paper spray ionization coupled with tandem mass spectrometry experiments, which allowed the recording of data very quickly and with high specificity. All investigated compounds gained a good linear relation (r2 higher than 0.98). Accuracy values are near 100% for all concentration levels examined, and the repeatability and reproducibility data result lower than 15%, highlighting the consistence of the methodology. The developed approach was successfully applied for the analysis of different real samples, and its robustness was confirmed by comparing the results obtained with those coming from the classical and official methodology.
2023
fatty acid methyl esters, vegetable oils, paper spray ionization, tandem mass spectrometry
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11770/343867
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 5
  • ???jsp.display-item.citation.isi??? ND
social impact