A new Molecularly Imprinted Solid Phase Extraction (MISPE) protocol was developed for the selectiveextraction and purification of glycyrrhizic acid (GL) from liquorice roots. Non-covalent MIP were synthesizedusing methacrylic acid (MAA), 2-(dimethylamino)ethyl methacrilate (DMAEM) or 2-hydroxyethylmetacrylate(HEMA) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as crosslinkingagent.After the evaluation of the selectivity of the GL imprinted polymers, the performance of these materialsas Solid Phase Extraction (SPE) sorbents was investigated. MIP having HEMA as functional monomer werefound to be able to selectively extract almost 80% of GL content in liquorice roots. The proposed MISPEHPLCprocedure has good precision, thus it can be successfully used for the purification of GL from naturalsources.
Molecularly imprinted polymers for the selective extraction of glycyrrhizic acid from liquorice roots
Cirillo G;Curcio M;Parisi OI;PUOCI F;IEMMA F;Spizzirri UG;RESTUCCIA D;PICCI N
2011-01-01
Abstract
A new Molecularly Imprinted Solid Phase Extraction (MISPE) protocol was developed for the selectiveextraction and purification of glycyrrhizic acid (GL) from liquorice roots. Non-covalent MIP were synthesizedusing methacrylic acid (MAA), 2-(dimethylamino)ethyl methacrilate (DMAEM) or 2-hydroxyethylmetacrylate(HEMA) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as crosslinkingagent.After the evaluation of the selectivity of the GL imprinted polymers, the performance of these materialsas Solid Phase Extraction (SPE) sorbents was investigated. MIP having HEMA as functional monomer werefound to be able to selectively extract almost 80% of GL content in liquorice roots. The proposed MISPEHPLCprocedure has good precision, thus it can be successfully used for the purification of GL from naturalsources.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
