Carabid beetles are among the most important groups of beneficial arthropods in the agroecosystem food chain where they are predators of many pests (including aphids, lepidopterans, slugs and Diptera). Previous studies have been shown that they are good models to investigate the negative effects of agrochemical used in agricultural management practices on natural enemies of insect pests. In ecological immunology, variation on immune capacity of insects is an early warning, highly sensitive biomarker to monitor the sub-lethal effect of toxicants introduced into environment as a result of industrial or agricultural activity. However, morpho-functional data about immune system of carabid are scanty in spite of their ecological relevance. In this study, we have investigated the immune function of Harpalus (Pseudoophonus) rufipesfor their use in eco-toxicological monitoring. This species is a omnivorous predator, very common in Calabrian (South Italy) agroecosystems and acts asa predator against insect pests. Tests performed on adult involve a general screen of cellular responses and include: characterization of circulating hemocytes and phagocytosis in vivo. The cellular population has been characterized by light and electron microscopy analysis and 4 morphotypes of circulating hemocytes were found: prohemocytes (0.55±0.11%), plasmatocytes (67±2.52%), granulocytes (29.32±2.35%), oenocytoids (0.73±0.20%). The phagocytosis assays were performed in vivoby injection of 0.9 μm carboxylate-modified polystyrene latex beads in order to identify the hemocyte types involved in phagocytosis. After 2h non-self challenge treatment, specimens showed a decrease of plasmatocytes and oenocytoid percentages (12.38±2.21% and 0.16±0.05%, respectively) and a non-specific immune response involving phagocytosis performed by granulocytes (82.74±2.54%). Moreover, hemocytes with mitotic figures and non-differentiate cells were found in the hemolymph (2.28±0.37), thus confirming a continuously turnover . Melanotic nodules have been found 2h after the immune challenge formed to immobilize the latex beads.
Cellular immune response in Harpalus (Pseudoophonus) runpes (De Geer, 1774) (Coleoptera, Carabidae). XXth scientific meeting of the Italian Association of Developmental and Comparative Immunobiology (IADCI)
A Giglio;F Cavaliere;F Talarico;ML Vommaro
;P Brandmayr
2019-01-01
Abstract
Carabid beetles are among the most important groups of beneficial arthropods in the agroecosystem food chain where they are predators of many pests (including aphids, lepidopterans, slugs and Diptera). Previous studies have been shown that they are good models to investigate the negative effects of agrochemical used in agricultural management practices on natural enemies of insect pests. In ecological immunology, variation on immune capacity of insects is an early warning, highly sensitive biomarker to monitor the sub-lethal effect of toxicants introduced into environment as a result of industrial or agricultural activity. However, morpho-functional data about immune system of carabid are scanty in spite of their ecological relevance. In this study, we have investigated the immune function of Harpalus (Pseudoophonus) rufipesfor their use in eco-toxicological monitoring. This species is a omnivorous predator, very common in Calabrian (South Italy) agroecosystems and acts asa predator against insect pests. Tests performed on adult involve a general screen of cellular responses and include: characterization of circulating hemocytes and phagocytosis in vivo. The cellular population has been characterized by light and electron microscopy analysis and 4 morphotypes of circulating hemocytes were found: prohemocytes (0.55±0.11%), plasmatocytes (67±2.52%), granulocytes (29.32±2.35%), oenocytoids (0.73±0.20%). The phagocytosis assays were performed in vivoby injection of 0.9 μm carboxylate-modified polystyrene latex beads in order to identify the hemocyte types involved in phagocytosis. After 2h non-self challenge treatment, specimens showed a decrease of plasmatocytes and oenocytoid percentages (12.38±2.21% and 0.16±0.05%, respectively) and a non-specific immune response involving phagocytosis performed by granulocytes (82.74±2.54%). Moreover, hemocytes with mitotic figures and non-differentiate cells were found in the hemolymph (2.28±0.37), thus confirming a continuously turnover . Melanotic nodules have been found 2h after the immune challenge formed to immobilize the latex beads.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
